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KMID : 0380620100420020223
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Korean Journal of Food Science and Technology
2010 Volume.42 No. 2 p.223 ~ p.232
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Enhancement of Immunostimulation by Fractionation of Active Polysaccharide from Fermented Ginseng with Phellinus linteus Mycelium in Solid Culture
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Kim Hoon
Yoon Hyun-Seok
Jeong Jae-Hyun
Jeong Heon-Sang
Hwang Jong-Hyun
Yu Kwang-Wan
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Abstract
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Crude polysaccharide (WG-PL-CP) was fractionated from fermented ginseng with Phellinus linteus in solid culture to enhance the immunostimulation of ginseng. WG-PL-CP produce three active polysaccharide-rich fractions (WGPL- CP-II, III, and IV) on DEAE-Sepharose CL-6B (Cl? form). WG-PL-CP-III displayed higher mitogenic activity (1.98- fold of the saline control at 100 μg/mL) than did WG-CP-III or PL-CP-III (1.60- or 1.65-fold, respectively), and potent intestinal immune system modulating activity through Peyer’s patch was obtained by WG-PL-CP-IV only (1.56-fold). Meanwhile, WG-PL-CP-II and III significantly enhanced macrophage stimulating activity (2.01- and 1.94-fold) compared to WG-CP-II and III (1.73- and 1.66-fold) or PL-CP-II and III (1.79- and 1.72-fold). In addition, WG-PL-CP-III and IV mainly contained neutral sugar (73.5 and 67.3%) and uronic acid (23.2 and 24.6%). Component sugar analysis also showed that WG-PL-CP-III consisted mainly of uronic acid as well as the neutral sugars Glc, Ara, Gal, Rha and Xyl (molar ratio of 0.81:1.00:0.49:0.42:0.28:0.20), whereas WG-PL-CP-IV was mainly comprised of uronic acid, Ara, Rha, Gal, Xyl and Glc (1.00:0.75:0.69:0.63:0.42:0.34). Therefore, it is assumed that these active polysaccharides play an important role in enhancing the immunostimulation of fermented ginseng with P. linteus in solid culture.
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KEYWORD
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Phellinus linteus, Korean ginseng, solid culture, immunostimulation, polysaccharide
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